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Literature summary extracted from

  • Ono, K.; Jung, M.; Zhang, T.; Tsutsuki, H.; Sezaki, H.; Ihara, H.; Wei, F.; Tomizawa, K.; Akaike, T.; Sawa, T.
    Synthesis of L-cysteine derivatives containing stable sulfur isotopes and application of this synthesis to reactive sulfur metabolome (2017), Free Radic. Biol. Med., 106, 69-79 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.144 gene cysM, recombinant expression of His-tagged enzyme in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.144 O-acetyl-L-serine + selenide Salmonella enterica subsp. enterica serovar Typhimurium low activity L-selenocysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + selenide Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 low activity L-selenocysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + selenide Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 low activity L-selenocysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + sulfide Salmonella enterica subsp. enterica serovar Typhimurium
-
 L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + sulfide Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412
-
 L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + sulfide Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
-
 L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + sulfite Salmonella enterica subsp. enterica serovar Typhimurium
-
 L-cysteine sulfonate + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + thiosulfate Salmonella enterica subsp. enterica serovar Typhimurium
-
 S-sulfo-L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + thiosulfate Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412
-
 S-sulfo-L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + thiosulfate Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
-
 S-sulfo-L-cysteine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.144 Salmonella enterica subsp. enterica serovar Typhimurium P29848
-
-
2.5.1.144 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 P29848
-
-
2.5.1.144 Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 P29848
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.144 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.144 O-acetyl-L-serine + thiosulfate = S-sulfo-L-cysteine + acetate ping pong bi bi kinetic mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.144 additional information development of a rapid and efficient method of synthesis of L-cysteine derivatives containing isotopic sulfur atoms and application of this method to a reactive sulfur metabolome of N-acetyl-L-cysteine (NAC) in HeLa cells. CysM catalyzes reaction with multiple substrates by a ping pong bi bi kinetic mechanism Salmonella enterica subsp. enterica serovar Typhimurium ?
-
-
2.5.1.144 additional information development of a rapid and efficient method of synthesis of L-cysteine derivatives containing isotopic sulfur atoms and application of this method to a reactive sulfur metabolome of N-acetyl-L-cysteine (NAC) in HeLa cells. CysM catalyzes reaction with multiple substrates by a ping pong bi bi kinetic mechanism Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 ?
-
-
2.5.1.144 additional information development of a rapid and efficient method of synthesis of L-cysteine derivatives containing isotopic sulfur atoms and application of this method to a reactive sulfur metabolome of N-acetyl-L-cysteine (NAC) in HeLa cells. CysM catalyzes reaction with multiple substrates by a ping pong bi bi kinetic mechanism Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 ?
-
-
2.5.1.144 O-acetyl-L-serine + selenide
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-selenocysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + selenide low activity Salmonella enterica subsp. enterica serovar Typhimurium L-selenocysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + selenide
-
 Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 L-selenocysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + selenide low activity Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 L-selenocysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + selenide
-
 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 L-selenocysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + selenide low activity Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 L-selenocysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + sulfide
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + sulfide
-
 Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + sulfide
-
 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + sulfite
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-cysteine sulfonate + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + thiosulfate
-
 Salmonella enterica subsp. enterica serovar Typhimurium S-sulfo-L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + thiosulfate
-
 Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 S-sulfo-L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + thiosulfate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 S-sulfo-L-cysteine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.144 CysM
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.144 cysteine synthase B UniProt Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.144 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.144 7.6
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium